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Literature summary for 3.4.24.89 extracted from

  • Klychnikov, O.I.; Shamorkina, T.M.; Weeks, S.D.; van Leeuwen, H.C.; Corver, J.; Drijfhout, J.W.; van Veelen, P.A.; Sluchanko, N.N.; Strelkov, S.V.; Hensbergen, P.J.
    Discovery of a new Pro-Pro endopeptidase, PPEP-2, provides mechanistic insights into the differences in substrate specificity within the PPEP family (2018), J. Biol. Chem., 293, 11154-11165 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.89

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta 2(DE3)pLysS cells Paenibacillus alvei

Crystallization (Commentary)

Crystallization (Comment) Organism
using 2.2 M (NH4)2SO4, 0.2 M CdSO4, 0.01 M ZnCl2 (pH 9.0) Paenibacillus alvei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 YPSSKPLPPVPPVQPLPPVPKLETS wild type enzyme PPEP-2, at pH 7.0 and 37°C Paenibacillus alvei
0.33
-
 YPSSKPLPPVPPVQPLPPVPKLETS recombinant enzyme PPEP-2GGST, where residues 112-115 of the wild type enzyme (SERV) are replaced by residues 91-94 of Pro-Pro endopeptidase-1 (GGST), at pH 7.0 and 37°C Paenibacillus alvei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Paenibacillus alvei the enzyme cleaves between two prolines in a PLPPVP motif ?
-
 ?
additional information Paenibacillus alvei ATCC 6344 the enzyme cleaves between two prolines in a PLPPVP motif ?
-
 ?

Organism

Organism UniProt Comment Textmining
Paenibacillus alvei K4ZRC1
-
-
Paenibacillus alvei ATCC 6344 K4ZRC1
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap SP column chromatography and HiTrapQ column chromatography Paenibacillus alvei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme cleaves between two prolines in a PLPPVP motif Paenibacillus alvei ?
-
 ?
additional information the enzyme poorly cleaves the VNPPVP peptide Paenibacillus alvei ?
-
 ?
additional information the enzyme cleaves between two prolines in a PLPPVP motif Paenibacillus alvei ATCC 6344 ?
-
 ?
additional information the enzyme poorly cleaves the VNPPVP peptide Paenibacillus alvei ATCC 6344 ?
-
 ?
YPSSKPLPPVPPVQPLPPVPKLETS + H2O
-
 Paenibacillus alvei YPSSKPLP + PVPPVQPLP + PVPKLETS
-
 ?
YPSSKPLPPVPPVQPLPPVPKLETS + H2O
-
 Paenibacillus alvei ATCC 6344 YPSSKPLP + PVPPVQPLP + PVPKLETS
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 20500, SDS-PAGE Paenibacillus alvei
monomer 1 * 21100, calculated from amino acid sequence Paenibacillus alvei

Synonyms

Synonyms Comment Organism
PPEP-2
-
 Paenibacillus alvei
Pro-Pro endopeptidase
-
 Paenibacillus alvei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2
-
 YPSSKPLPPVPPVQPLPPVPKLETS recombinant enzyme PPEP-2GGST, where residues 112-115 of the wild type enzyme (SERV) are replaced by residues 91-94 of Pro-Pro endopeptidase-1 (GGST), at pH 7.0 and 37°C Paenibacillus alvei
8
-
 YPSSKPLPPVPPVQPLPPVPKLETS wild type enzyme PPEP-2, at pH 7.0 and 37°C Paenibacillus alvei

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.061
-
 YPSSKPLPPVPPVQPLPPVPKLETS recombinant enzyme PPEP-2GGST, where residues 112-115 of the wild type enzyme (SERV) are replaced by residues 91-94 of Pro-Pro endopeptidase-1 (GGST), at pH 7.0 and 37°C Paenibacillus alvei
114.286
-
 YPSSKPLPPVPPVQPLPPVPKLETS wild type enzyme PPEP-2, at pH 7.0 and 37°C Paenibacillus alvei